Journal: Frontiers in Genetics

Article Title: Post-transcriptional regulation of BRCA1 through its coding sequence by the miR-15/107 group of miRNAs

doi: 10.3389/fgene.2015.00242

Figure Lengend Snippet: Putative miR-15/107 targets within the BRCA1 CDS. (A) Schematic representation of protein-coding BRCA1 transcript. (B) Density function and cumulative distribution of CDS length across all human coding mRNAs. (C) The rna22-predicted target site for the miR-15/107 group together with the corresponding miRNA:mRNA heteroduplexes.

Article Snippet: Primary antibodies include anti-BRCA1 (D54A8, Cell Signaling, Danvers, MA, USA) and anti-β-actin (8H10D10, Cell Signaling).

Techniques:

Journal: Frontiers in Genetics

Article Title: Post-transcriptional regulation of BRCA1 through its coding sequence by the miR-15/107 group of miRNAs

doi: 10.3389/fgene.2015.00242

Figure Lengend Snippet: Suppression of BRCA1 mRNA by miR-15/107 miRNAs. Cell lines transfected with miR-15/107 miRNA precursors show a significant decrease in the amount of available BRCA1 transcript 48 h post-transfection as detected by qRT-PCR. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001 compared to control scramble miR by Student’s t -test, n = 3 in each group.

Article Snippet: Primary antibodies include anti-BRCA1 (D54A8, Cell Signaling, Danvers, MA, USA) and anti-β-actin (8H10D10, Cell Signaling).

Techniques: Transfection, Quantitative RT-PCR, Control

Journal: Frontiers in Genetics

Article Title: Post-transcriptional regulation of BRCA1 through its coding sequence by the miR-15/107 group of miRNAs

doi: 10.3389/fgene.2015.00242

Figure Lengend Snippet: Characterization of a novel miR-15/107 target site within the BRCA1 CDS. The putative binding site for the miR-15/107 group in BRCA1’s CDS was cloned into a luciferase reporter vector along with a corresponding construct containing mutations within the putative miRNA ‘seed’ recognition sites to confirm specificity. ∗ P < 0.05, ∗∗ P < 0.01 compared to control scramble target sequence by Student’s t -test, n = 3 in each group.

Article Snippet: Primary antibodies include anti-BRCA1 (D54A8, Cell Signaling, Danvers, MA, USA) and anti-β-actin (8H10D10, Cell Signaling).

Techniques: Binding Assay, Clone Assay, Luciferase, Plasmid Preparation, Construct, Control, Sequencing

Journal: Frontiers in Genetics

Article Title: Post-transcriptional regulation of BRCA1 through its coding sequence by the miR-15/107 group of miRNAs

doi: 10.3389/fgene.2015.00242

Figure Lengend Snippet: Sequestration of miR-15/107 miRNAs impacts BRCA1 mRNA. Cells transfected with luciferase reporter plasmids containing antisense (as) miRNA sequences or predicted wild type (WT) BRCA1 miR-15/107 MRE demonstrate varying levels of BRCA1 expression rescue. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001 compared to control by Student’s t -test, n = 3 in each group.

Article Snippet: Primary antibodies include anti-BRCA1 (D54A8, Cell Signaling, Danvers, MA, USA) and anti-β-actin (8H10D10, Cell Signaling).

Techniques: Transfection, Luciferase, Expressing, Control

Journal: Frontiers in Genetics

Article Title: Post-transcriptional regulation of BRCA1 through its coding sequence by the miR-15/107 group of miRNAs

doi: 10.3389/fgene.2015.00242

Figure Lengend Snippet: Translational suppression of BRCA1 by miR-15/107 miRNAs. Western immunoblots from hTERT-HPNE, HCT-116, and MIA PaCa-2 cells demonstrate relative levels of BRCA1 protein expression at 72 h post-transfection with control scramble miRNA and anti-miR precursors, miRNA and anti-miR precursors, or siBRCA1 control.

Article Snippet: Primary antibodies include anti-BRCA1 (D54A8, Cell Signaling, Danvers, MA, USA) and anti-β-actin (8H10D10, Cell Signaling).

Techniques: Western Blot, Expressing, Transfection, Control